Sci Rep. 2017 Jul 26;7(1):6520. doi:10.1038/s41598-017-04765-8

Ohtsubo Y, Nagata Y, Tsuda M.

Compounds that enhance the tailing activity of Moloney murine leukemia virus reverse transcriptase.

rA, r/dG, r/dC are potent enhancers of tailing. In longer reactions, GMP, GDP or CDP promote continuous extension of the tail. Reactions performed with 100 fmols of substrate DNA, 50 mM Tris-HCl pH 8.3, 75 mM KCl, 6 mM MgCl2, 2 mM DTT, 4 mM dATP, dCTP, dGTP, or dTTP, 4 mM MnCl2, and 50 U MMLV-RT, at 30 °C.