Omotezako T, Matsuo M, Onuma TA, Nishida H

Sci Rep. 2017 Mar 10;7:44226. doi:10.1038/srep44226

DNA interference-mediated screening of maternal factors in the chordate Oikopleura dioica.

A clone library was prepared by subtracting male to female cDNAs. Clones were screened by pools of 5 and then resolved individually. Identifies genes related to cell structure and adhesion.