Svensson V, Natarajan KN, Ly LH, Miragaia RJ, Labalette C, Macaulay IC, Cvejic A, Teichmann SA.
Nat Methods. 2017 Apr;14(4):381-387. doi:10.1038/nmeth.4220
Power analysis of single-cell RNA-sequencing experiments.
Freeze-thaw cycles decrease detected amounts of ERCC spike molecules.