Kilili GK, Tilton L, Karbiwnyk CM.

Biotechniques. 2016 Sep 1;61(3):114-6. doi:10.2144/000114449

NaOH concentration and streptavidin bead type are key factors for optimal DNA aptamer strand separation and isolation.

High pH releases streptavidin protein and biotinylated probe as well as the hybridised target, especially with some brands.