Yokomori R, Shimai K, Nishitsuji K, Suzuki Y, Kusakabe TG, Nakai K.

Genome Res. 2016 Jan;26(1):140-50. doi:10.1101/gr.184648.114

Genome-wide identification and characterization of transcription start sites and promoters in the tunicate Ciona intestinalis.

TSS-Seq libraries made of 200 µg of total RNA, sequenced on Illumina GA with a read length of 36, which is sufficient to go through the 16-nt splice leader.