Seitz V, Schaper S, Dröge A, Lenze D, Hummel M, Hennig S.

Nucleic Acids Res. 2015 Nov 16;43(20):e135. doi:10.1093/nar/gkv694

A new method to prevent carry-over contaminations in two-step PCR NGS library preparations.

Proposes to introduce indexes during the cDNA PCR, and to partially match them during Library PCR, so that 1) the PCR is more stringent and 2) contaminations can be detected by sequence analysis.