Xu H, Sepúlveda LA, Figard L, Sokac AM, Golding I.

Nat Methods. 2015 Aug;12(8):739-742. doi:10.1038/nmeth.3446

Combining protein and mRNA quantification to decipher transcriptional regulation.

Analysis of > 21,000 loci from 31 drosophila embryos. Cooperative binding of 6 Bcd molecules to the hb locus. Even at highest Bcd concentration, the gene is inactive roughly half of the time.