Zhu Y, Zhang YX, Liu WW, Ma Y, Fang Q, Yao B.

Sci Rep. 2015 Apr 1;5:9551. doi:10.1038/srep09551

Printing 2-dimentional droplet array for single-cell reverse transcription quantitative PCR assay with a microfluidic robot

For the amplification of a miRNA, the authors reported a 98.03% efficiency in standard conditions. It dropped to 90.94% with 0.5 mM 49.99% PBS, 86.96% with 2 mM, 80.47% with 10 mM (1×), and 49.99% with 50 mM. The PCR was done in TaqMan universal PCR master mix.