Larman HB, Scott ER, Wogan M, Oliveira G, Torkamani A, Schultz PG

Nucleic Acids Res. 2014 Oct 1;42(14):9146-57. doi: 10.1093/nar/gku636

Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay.

Decoy probes to reduce the number of reads for the most highly expressed genes. Uses T4 Rnl2, which was superior in consistency to splintR. Ligation validated by capillary sequencing. When hybridizing oligonucleotides to a target to form a nicked double strand, annealing is more efficient if the 5′ end at the nick is not phosphorylated.