Machida RJ, Lin YY.

PLoS One. 2014 Jul 8;9(7):e101812. doi: 10.1371/journal.pone.0101812

Four methods of preparing mRNA 5' end libraries using the illumina sequencing platform.

Capture of 5′ ends via biotinylated forward PCR primers. CapSMART: ligation of modified oligonucleotides that block RT on 5′-phoshphorylated RNAs (after kinase treatment). The signal is not much different from standard SMART.