RNA Biol. 2014;11(7):817-28. doi:10.4161/rna.29304

Turchinovich A, Surowy H, Serva A, Zapatka M, Lichter P, Burwinkel B.

Capture and Amplification by Tailing and Switching (CATS). An ultrasensitive ligation-independent method for generation of DNA libraries for deep sequencing from picogram amounts of DNA and RNA.

Pre-treatment by T4 PNK was needed when using circulating plasma RNA, probably because of phosphate or cyclo-phosphates at the 3′ end (caused by RNA cleavage). RT primer concentration scaled down according to the RNA amoounts (0.1 µM for 1 ng and 0.1 nM for 5 pg). TSO concentration: 1 µM.