Poh YC, Chen J, Hong Y, Yi H, Zhang S, Chen J, Wu DC, Wang L, Jia Q, Singh R, Yao W, Tan Y, Tajik A, Tanaka TS, Wang N

Nat Commun. 2014 May 30;5:4000. doi:10.1038/ncomms5000

Generation of organized germ layers from a single mouse embryonic stem cell.

If only cultured in 3D soft fibrin gels, embryoid bodies develop germ layers, but with mesoderm outside and ectoderm inside.