Fu GK, Xu W, Wilhelmy J, Mindrinos MN, Davis RW, Xiao W, Fodor SP.

Proc Natl Acad Sci U S A. 2014 Feb 4;111(5):1891-6. doi: 10.1073/pnas.1323732111

Molecular indexing enables quantitative targeted RNA sequencing and reveals poor efficiencies in standard library preparations.

Estimates overall efficiency to be 0.1 % or even lower. 96 × 96 barcodes ligated to cDNA fragments.