Anal Chem. 2012 Apr 17;84(8):3599-606.

Zhang H, Jenkins G, Zou Y, Zhu Z, Yang CJ.

Massively parallel single-molecule and single-cell emulsion reverse transcription polymerase chain reaction using agarose droplet microfluidics.

Primers bound to agarose by 5′ acrydite residues. RT and PCR chained in the same reaction mixture using hot start PCR enzyme. Agarose beads sorted by FACS.