Jen CP, Hsiao JH, Maslov NA.

Sensors (Basel). 2012;12(1):347-58. doi: 10.3390/s120100347

Single-cell chemical lysis on microfluidic chips with arrays of microwells.

Lyses within 12 s with a strong lysis buffer containing 25 mM Tris pH 8, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1 % Triton X-100, 2.5 mM sodium pyrophoshpate, 1 mM beta-glycerolphosphate and 2 mM phenylmethylsulfonyl fluoride (PMSF).