Von Keyserling H, Bergmann T, Wiesel M, Kaufmann AM.

Biotechniques. 2011 Sep;51(3):179-84. doi:10.2144/000113735

The use of melting curves as a novel approach for validation of real-time PCR instruments.

Variations between technical replicates in melting temperature evaluated with melting curve analysis may identify heating biases of the instrument.