Biotechniques. 2011 Mar;50(3):177-80.

Method for improved Illumina sequencing library preparation using NuGEN Ovation RNA-Seq System.

Head SR, Komori HK, Hart GT, Shimashita J, Schaffer L, Salomon DR, Ordoukhanian PT.

Digests possible terminal hairpins with nuclease S1, in order to increase yield of blunting and end repair step.