Chez Charles/ biblio/ An improved one-tube RT-PCR protocol for analyzing single-cell gene expression in individual mammalian cells. Edit RecentChanges History Preferences Branchable Cell lysis buffer: RNase inhibitor: 2 U/μl, NaCl: 0.135 M, Tris-HCl (pH 8): 9 mM, DTT: 4.5 mM