Stoeckius M, Maaskola J, Colombo T, Rahn HP, Friedländer MR, Li N, Chen W, Piano F, Rajewsky N.

Nat Methods. 2009 Oct;6(10):745-51. doi:10.1038/nmeth.1370

Large-scale sorting of C. elegans embryos reveals the dynamics of small RNA expression.

“embryos were fixed by resuspending them in −20 °C methanol (80%). The tube was then placed on an overhead rotator at 4 °C for at least 1 h”