Armour CD, Castle JC, Chen R, Babak T, Loerch P, Jackson S, Shah JK, Dey J, Rohl CA, Johnson JM, Raymond CK.

Nat Methods. 2009 Sep;6(9):647-9. doi:10.1038/nmeth.1360

Digital transcriptome profiling using selective hexamer priming for cDNA synthesis.

Because of reduced complexity, many 5′ ends are difficult to cover.