Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM.

PLoS One. 2009 Jul 27;4(7):e6372. doi:10.1371/journal.pone.0006372

A method for studying protistan diversity using massively parallel sequencing of V9 hypervariable regions of small-subunit ribosomal RNA genes.

Primers contain degenerate sequences and are either universal or eukaryote-based. Samples from Mount Hope Bay, Massachusetts and Palmer Station, Antarctica.

>18S_1380F eukaryotic 10.1371/journal.pone.0006372
CCCTGCCHTTTGTACACAC
>18S_1389F universal 10.1371/journal.pone.0006372
TTGTACACACCGCCC
>18S_1510R eukaryotic 10.1371/journal.pone.0006372
CCTTCYGCAGGTTCACCTAC