Morin R, Bainbridge M, Fejes A, Hirst M, Krzywinski M, Pugh T, McDonald H, Varhol R, Jones S, Marra M.

Biotechniques. 2008 Jul;45(1):81-94. doi:10.2144/000112900

Profiling the HeLa S3 transcriptome using randomly primed cDNA and massively parallel short-read sequencing.

Tempate switching and RT with random hexamers, followed by sonication, adapter ligation, size fractionnation, amplification and sequencing.