Bontoux N, Dauphinot L, Vitalis T, Studer V, Chen Y, Rossier J, Potier MC.

Lab Chip. 2008 Mar;8(3):443-50

Integrating whole transcriptome assays on a lab-on-a-chip for single cell gene profiling.

For simple RT-PCR, cDNAs were pre-amplified 40× on chip. cDNAs must be dilluted at least 500× before amplification, otherwise TS-PCR is inhibited by leftovers of the RT.