Biotechniques. 2006 Apr;40(4):469-70, 472, 474

Ozawa T, Kishi H, Muraguchi A.

Amplification and analysis of cDNA generated from a single cell by 5'-RACE: application to isolation of antibody heavy and light chain variable gene sequences from single B cells.

50 % success on B cells to amplify the Ig variable region with polyG tailing and nested PCR.