Salk JJ, Sanchez JA, Pierce KE, Rice JE, Soares KC, Wangh LJ.

Anal Biochem. 2006 Jun 1;353(1):124-32 doi:10.1016/j.ab.2006.02.012

Direct amplification of single-stranded DNA for pyrosequencing using linear-after-the-exponential (LATE)-PCR.

The PPi produced during the PCR can be removed with the pyrosequencing enzymes themselves. The antarctic phosphatase from NEB was not very efficient at this task.