Suslov O, Steindler DA.

Nucleic Acids Res. 2005 Nov 27;33(20):e181.

PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency.

Phenol / Chloroform / Isoamyl alcohol (PCI) treatment followed by ethanol precipitation strongly decreases Ct as well as increases the consistencies of the comparisons between different dillutions of the same samples