J Biosci Bioeng. 2005 Mar;99(3):293-5. doi:10.1263/jbb.99.293

Nakano M, Nakai N, Kurita H, Komatsu J, Takashima K, Katsura S, Mizuno A.

Single-molecule reverse transcription polymerase chain reaction using water-in-oil emulsion.

The emulsion is destroyed by centrifugation after 13 cycles, and then the PCR is restarted for 30 cycles.