Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11701-6. doi:10.1073/pnas.0403514101
Wei CL, Ng P, Chiu KP, Wong CH, Ang CC, Lipovich L, Liu ET, Ruan Y.
5' Long serial analysis of gene expression (LongSAGE) and 3' LongSAGE for transcriptome characterization and genome annotation.
81% of the 5'/3' tag pairs could be used to amplify a cDNA by RT-PCR.