Science. 1992 Nov 13;258(5085):1112-8 doi:10.1126/science.1279806

Peliska JA, Benkovic SJ.

Mechanism of DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase.

Addition of 1 extra nucleotide happens at an apparent rate of 0.1 / min. Addition of subsequent bases is visible (Fig 3) but much slower. This addition does not requrie RNAseH activity. A > G > T > C ratio on a non-capped oligonucleotide duplex: 1 : 0.5 : 0.2 : 0.07, determined by incubating the reaction with each individual dNTP. 50 mM Tris-HCl pH 8, 75 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 200 nM DNA/RNA duplex, each dNTP at 100 µM, 7 mM MgCl2, 800 nM TSO and 200 nM HIV-1 RT, at 37 °C.