Mol Biotechnol. 2002 Nov;22(3):223-30 doi:10.1385/MB:22:3:223

Reddy MK, Nair S, Sopory SK.

Global amplification of cDNA from limiting amounts of tissue. An improved method for gene cloning and analysis.

Ligating a DNA adaptor 3′ to the FScDNA using T4 RNA ligase.