Chez Charles/ biblio/ Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions.

Lekanne Deprez RH, Fijnvandraat AC, Ruijter JM, Moorman AF.

Anal Biochem. 2002 Aug 1;307(1):63-9.

Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions.

DTT interferes with fluorescence measurements in qPCR. RT without DTT is efficient. Random-primerd replicates are less reproducible than oligo-dT or gene-specific-primed ones.