Biotechniques. 2001 Mar;30(3):574-80, 582

Chen D, Patton JT.

Reverse transcriptase adds nontemplated nucleotides to cDNAs during 5'-RACE and primer extension.

Terminal desoxynucleotidyl transferase activity on double-stranded substrates of reverse transcriptases MMLV and AMV: preference for adding As. For MMLV, activity reduced abruptly between 45 and 50 °C. For AMV, it decreased constantly from 25 to 50 °C. Activity increased with concentration for MMLV. (High-concentration AMV was not available.)