Nucleic Acids Res. 2001 Mar 1;29(5):E29

Baugh LR, Hill AA, Brown EL, Hunter CP.

Quantitative analysis of mRNA amplification by in vitro transcription.

T7 pol generates aberrant template-unrelated products. This is fixed by reducing the concentration of oligo dT primers, and of enzymes. Under these conditions, 2 rounds of RNA linear amplification is not generatng too much bias. Small-volume protocol. T4gp32, a single stranded protein, increases RT processivity.