Shi X, Kaminskyj SG.

Biotechniques. 2000 Dec;29(6):1192-5 doi:10.2144/00296bm07

5' RACE by tailing a general template-switching oligonucleotide.

Generate a collection of TSOs by TdT tailing DNA oligos with GTP. As the lengh of the G tail is variable, it is thought to be more suited to the template switching on cDNAs where the C tail is also variable in length.