Huxley, Thomas Henry.

Philosophical Transactions of the Royal Society of London, vol. 141, 1851, pp. 595–605.

Remarks upon Appendicularia and Doliolum, Two Genera of the Tunicata

https://www.jstor.org/stable/108414

Observed appendicularians in New Guinea and in the South Pacific. Found testis but no ovary. Sampled animals did not have their house. Recognised that Appendicularians are Tunicates.