Atsuo Nishino, and Masaaki Morisawa

ZOOLOGICAL SCIENCE 15: 723–727 (1998) doi:10.2108/zsj.15.723

Rapid Oocyte Growth and Artificial Fertilization of the Larvaceans Oikopleura dioica and Oikopleura longicauda

Oikopleura (dioica and longicauda) sampled from Moroiso bay, Misaki, Miura peninsula and cultivated at 18 °C. In O. dioica, the first polar body is visible 5 min after fertilisation and the second polar body 15 min after fertilisation. O. longicauda individuals can self-fertilise. F. pellucida could not be in vitro fertilised with the same method.