Esben Bjoern Madsen, Thomas Kvist, Ida Hoijer, Adam Ameur, Marie Just Mikkelsen

bioRxiv, September 6, 2018.

Xdrop: targeted sequencing of long DNA molecules from low input samples using droplet sorting

Enrichment by 1) encapsulating DNA fragments in double-emulsion water-in-oil-in-water droplets, 2) sorting the droplets containing the target sequence detected by a hydrolysis probe PCR, 3) extracting DNA from the droplets, 4) amplifying it by MDA (in a second generation of droplets), and finally 5) sequencing. When enriching for HPV DNA in HeLa genomes, 5 to 10% of the reads were on target. Emulsions were prepared in Dolomite chips.