Nejc Haberman, Holly Digby, Rupert Faraway, Rebecca Cheung, Andrew M Jobbins, Callum Parr, Kayoko Yasuzawa, Takeya Kasukawa, Chi Wai Yip, Masaki Kato, Hazuki Takahashi, Piero Carninci, Santiago Vernia, Jernej Ule, Christopher R Sibley, Aida Martinez-Sanchez, Boris Lenhard

bioRxiv 2023.04.27.538568; doi:10.1101/2023.04.27.538568

Abundant capped RNAs are derived from mRNA cleavage at 3’UTR G-Quadruplexes

3′ peaks colocalising with a G-rich motif are now explained by AGO2-mediated cleavage of G-quadruplex regions and recapping. The 3′ fragments and the mRNA did not localise equally in the cell when imaged by in-situ hybridisation.