Kim Y, Tanner HM, Rosenthal JJC, Brangwynne CP

STAR Protoc. 2025 Sep 19;6(3):103994. doi:10.1016/j.xpro.2025.103994

Protocol for the isolation, culture, and transfection of squid primary cells

Fibroblast-like cells migrate out of optic lobe, eye or gill explants after trypsin digestion, and can be transfected, but no cell division was observed. Insulin-transferrin-selenium (ITS-G), FGF and EGF are present in the cell culture medium.