Chez Charles/ biblio/ Differential priming of RNA templates during cDNA synthesis markedly affects both accuracy and reproducibility of quantitative competitive reverse-transcriptase PCR.

Zhang J1, Byrne CD.

Biochem J. 1999 Jan 15;337 ( Pt 2):231-41. doi:10.1042/bj3370231

Differential priming of RNA templates during cDNA synthesis markedly affects both accuracy and reproducibility of quantitative competitive reverse-transcriptase PCR.

cDNA yield did not increase with higher concentrations of specific hexamers. Higher yields with random hexamers. No data for reverse-transcription without primers.