Lee YH, Hsueh YW, Peng YH, Chang KC, Tsai KJ, Sun HS, Su IJ, Chiang PM.

BMC Biol. 2017 Mar 21;15(1):22. doi:10.1186/s12915-017-0359-5

Low-cell-number, single-tube amplification (STA) of total RNA revealed transcriptome changes from pluripotency to endothelium.

Poly-A tailing followed by template switching. Increased dNTPs to 2 mM and Mg2+ to 9 mM to favour terminal addition of nucleotides. TSO and RT primers are at 1 μM final.